Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1.
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منابع مشابه
Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1.
The chromosomal mes 1 mutation appears to elevate the Km of methionine for yeast methionyl-tRNA synthetase. The mutation was cloned on a multicopy plasmid by gap repair of a plasmid bearing the wild type MES1 gene for a fragment corresponding to the mes 1 mutation. DNA sequencing established that the mutation consists of a single conversion of guanine into adenine which results in the replaceme...
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Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)48142-2